Journal: Cell

Article Title: Lipid Biosynthesis Coordinates a Mitochondrial-to-Cytosolic Stress Response

doi: 10.1016/j.cell.2016.08.027

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cell Culture and Maintenance Adult human dermal fibroblasts (Lonza) were cultured in DMEM, 10% FBS and 1× GlutaMAX (GIBCO) supplemented with blasticidin (when appropriate, 2 ug/mL for selection and 1 ug/mL for maintenance of transduced cell lines).

Techniques: Recombinant, Microarray, Sequencing, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls

doi: 10.1371/journal.pone.0108336

Figure Lengend Snippet: (a) Quantification of ABCC6 mRNA expression in human dermal fibroblasts from healthy controls (n = 5; white) and PXE patients (n = 6; black). Effect of siRNA-mediated knockdown on ABCC6 gene expression: fibroblasts transfected with a scramble siRNA-negative control (siNK, n = 3; white); ABCC6-specific siRNA-treated cells (siABCC6, n = 3; black). Expression levels are normalized to reference gene expressions (ACTB, GAPDH, ß2M). Data are presented in arbitrary units as means with corresponding standard error. Differences between controls and PXE fibroblasts, as well as between siRNA-transfected cells, were analyzed using unpaired t-test with Welch's correction. (b) Western Blot analysis of ABCC6 in fibroblasts of healthy controls, PXE patients and siRNA- treated cells. Western blot analysis was performed with pooled protein samples from each group, with GAPDH as normalization control. HEK-293 cell lysates were used as an additional blotting control. (c) Representative Western Blot for fibroblasts of healthy controls, PXE patients and (d) siRNA- treated cells.

Article Snippet: Dermal fibroblasts from six healthy controls (NHDF) were purchased from Promocell (Heidelberg, Germany), Genlantis (San Diego, USA), Cambrex (Walkersville, USA) and Coriell (New Jersey, USA).

Techniques: Expressing, Knockdown, Gene Expression, Transfection, Negative Control, Western Blot, Control

Journal: PLoS ONE

Article Title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls

doi: 10.1371/journal.pone.0108336

Figure Lengend Snippet: Summary of metabolic findings in PXE human dermal fibroblasts compared to healthy controls.

Article Snippet: Dermal fibroblasts from six healthy controls (NHDF) were purchased from Promocell (Heidelberg, Germany), Genlantis (San Diego, USA), Cambrex (Walkersville, USA) and Coriell (New Jersey, USA).

Techniques:

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Resveratrol-Enriched Rice Attenuates UVB-ROS-Induced Skin Aging via Downregulation of Inflammatory Cascades

doi: 10.1155/2017/8379539

Figure Lengend Snippet: The levels of ROS in NHDFs treated as indicated for 24 h were measured using flow cytometry with DCFH-DA dye. The number of cells is plotted against the dichlorofluorescein fluorescence detected by the FL-1 channel (a). The relative ROS production of the cells is shown in each histogram (b). Values are mean ± SEM. The labels # and ∗ indicate significant differences ( p < 0.05) when compared with the normal control and UV (+) control, respectively. ### p < 0.001 versus the normal control, ∗∗ p < 0.01 versus the UVB-irradiated control. NC is normal control, Ctl is UVB control, NR is normal rice, RR is resveratrol-enriched rice, and R is resveratrol. NR and RR were treated in μ g/mL, and R was treated in μ M.

Article Snippet: Normal human dermal fibroblast cells (NHDFs) were obtained by skin biopsy from a healthy young male donor (MCTT Core Inc., Seoul, Korea).

Techniques: Flow Cytometry, Fluorescence, Control, Irradiation

Journal: International Journal of Molecular Sciences

Article Title: A New Bone Substitute Developed from 3D-Prints of Polylactide (PLA) Loaded with Collagen I: An In Vitro Study

doi: 10.3390/ijms18122569

Figure Lengend Snippet: Microscopic analysis of different cell types seeded on PLA discs. ( A ) hOB (human primary osteoblasts; ( B ) normal human dermal fibroblasts: NHDF; ( C ) human umbilical vein endothelial cells: HUVEC; and ( D ) osteosarcoma cells: SaOS-2. Scale bars represent 400 µm.

Article Snippet: Normal human dermal fibroblasts (NHDF, Promega, Karlsruhe, Germany), human primary osteoblasts (hOB [ , ]), osteoblast-like cells (SaOS, ATCC, Manassas, VA, USA) and endothelial cells (HUVEC, Promega, Karlsruhe, Germany) were seeded onto discs, into or onto cages, respectively, sitting in a 24-well ultra-low attachment plate.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A New Bone Substitute Developed from 3D-Prints of Polylactide (PLA) Loaded with Collagen I: An In Vitro Study

doi: 10.3390/ijms18122569

Figure Lengend Snippet: Proliferation assays on PLA discs with different cell types. ( A ) without collagen; ( B ) PLA discs coated with rat tail collagen; ( C ) PLA discs coated with bovine collagen. Blue: HUVEC, red: NHDF, green: hOB and lilac: SaOS-2.

Article Snippet: Normal human dermal fibroblasts (NHDF, Promega, Karlsruhe, Germany), human primary osteoblasts (hOB [ , ]), osteoblast-like cells (SaOS, ATCC, Manassas, VA, USA) and endothelial cells (HUVEC, Promega, Karlsruhe, Germany) were seeded onto discs, into or onto cages, respectively, sitting in a 24-well ultra-low attachment plate.

Techniques: